small intestine has the paradoxical
dual function of being a digestive
and absorptive organ for nutrients
as well as a powerful barrier against
the excessive absorption of bacteria,
food antigens and large molecules. Increased
permeability of the intestinal mucosal
barrier can swell the number of
toxins & antigens entering the
bloodstream and lead to an overly
sensitized immune system in some
individuals. Decreased permeability,
on the other hand, appears as a fundamental
cause of malnutrition, malabsorption
and failure to thrive. A number
of clinical disorders are associated
with both conditions.
The Intestinal Antigenic Permeability Screen by Cyrex Labs
MEASURING INTESTINAL PERMEABILITY
Two decades ago, the assessment of Intestinal Permeability was done by measuring the leakage of a sugar molecule (lactulose) into the blood dtream. Since then, numerous studies have questioned the clinical relevance of this marker. IT is not measuring antigenic macromolecule passage, because passage of very small molecules such as sugar, lacks a capacity to challenge the immune system. Therefore, measurements of intestinal permeability to antigenic molecules are assessed not only against the triggers of intestinal barrier degradation such as bacterial endotoxins, but also against barrier structures occulin/zonulin and actomyosin, which represents both paracellular and transcellular pathways.
Therefore, macromolecular intestinal permeability, which stimulates an immune response, can be accurately measured by identifying either antibodies to antigenic molecules, which trigger intestinal barrier degradation such as bacterial endotoxins, or antibodies against the componets involved in the intestinal barrier degradation such as occudin/zonulin and actomyosin.
Elevated antibodies to LPS, (Lipopolysaccharides), Occuludin/Zonulin and the Actomyosin Network are patent pending biomarkers that identigy the breakdown of a healthy intestinal barrier, which allow penetration of large antigenic molecules.
1. LPS (Lipopolysaccharides) are large molecules found in gram-negative bacteria. They are endotoxins,mand if absorbed, elicit a strong immune response. The dection of antibodies against LPS reveals macromolecule-sized endotoxin infiltration through the intestinal barrier into the systemic circulation.
2. Occulin is part of the main component of proteins holding together the tight junctions. The detection of antibodies to occludin indicates that the tight junctions are breaking down. This is a measure of a mecanism involved in damaging the intestinal membrane.
3. Zonulin, a protein regulates the permeability of the intestine. The detection of antibodies against zonulin indicates that the normalregulation of tight junctions is compromised. This can be a clue to presence of an ongoing mechanism involved in damaging the inttestinal barier.
4. The Actomyosin Network, a protein complex, regulates intestinal barrier function by maintaining the plasticity of tight junctions. Antibodies to the actomyosyn network are a biomarker of intestinal barrier dysregulation via cell infiltration. For example,m 98.2% of Celiac disease patients with flat mucosa have antibodies to actin. This is a measure of mechanism involved in damaging the intestinal barrier.
Antibody Array 2 for assessment of intestinal barrier integrity measures antibodies against bacterial endotoxins (lipopolusaccharides), tight-junction proteins (occuluin, zonulin) and cell cytoskeleton (actomyosin), and identifies both transcellular and paracellular routes of intestinal penetration by large molecules with a capacity to challenge the immune system.
Call to set up a nutritional consultation so that tests can be performed
and a comprehensive strategy of lifestyle, dietary modification
and nutrient supplementation can be implemented to aid you in reversing
Rispoli has her Ph.D. in Nutrition,
is a licensed Acupuncturist and
clinical researcher. She has been
in practice for well over 25 years.
It is through her clinical practice
that she has gained insights into
chronic health conditions. If
you are suffering from unexplained
symptoms that come and go you owe
it to yourself to find out why.
Find out today call for a Nutritional
Consultation with Dr. Rispoli.
Wishing you wellness.
an appointment, contact her office
at (800) 956-7083 or (818) 707-3125.
We also offer Functional Laboratory
tests that can be done through
the mail in the privacy of your
home to help determine why you
are having these symptoms. For
more information on these click
on lab tests.
The information herein is not intended
as diagnosis, treatment or a cure.
Should you have a medical condition
please seek the advice of your medical